Are fluorescence-detected sedimentation velocity data reliable?
نویسندگان
چکیده
منابع مشابه
Accounting for Photophysical Processes and Specific Signal Intensity Changes in Fluorescence-Detected Sedimentation Velocity
Fluorescence detected sedimentation velocity (FDS-SV) has emerged as a powerful technique for the study of high-affinity protein interactions, with hydrodynamic resolution exceeding that of diffusion-based techniques, and with sufficient sensitivity for binding studies at low picomolar concentrations. For the detailed quantitative analysis of the observed sedimentation boundaries, it is necessa...
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ANDERSON, P. M., AND MEISTER, A. (1965) Biochemistry 4, 2803-2809 ANDERSON, P. M., AND MEISTER, A. (1966) Biochemistry 6, 3164-3169 PI~RARD, A. (1966) Science 164, 1572-1573 ANDERSON, P. M., AND MARVIN, S. V. (1968) Biochem. Biophys. Res. Commun. 32, 92&934 PIBRARD, A., GLANSDORFF, N., MERGEAY, M., AND WIAME, J. M. (1965) J. Mol. Biol. 14, 23-36 ANDERSON, P. M., AND MEISTER, A. (1966) Biochemis...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2013
ISSN: 0003-2697
DOI: 10.1016/j.ab.2013.02.019